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Resting cell experiments.

Bacteria were grown for 2 days (1 day for Alcaligenes faecalis) as described above in PO-YE medium and then were harvested by centrifugation at 0 to 4℃.

細菌は、PO-YE培地で2日(Alcaligenes faecalisは1日)増殖させ、0~4℃の遠心分離により回収した。

The cells were washed twice with cold mineral salts mixture (lacking C and N compounds) and finally resuspended in an appropriate volume of the same mixture.

細胞は、冷えた無機塩類混合物(炭素と窒素は少量)と最終的な浮遊物は同じ適切な多量の混合物で洗った。

The determination of PO of NH2OH oxidation activity at 21 to 23℃ was as previously described (8), except that the initial concentration of PO or NH2OH was 2 mM.

PO、NH2OHの初期濃度が2mMであった以外、21℃~23℃までのNH2OH酸化活性のPO定量は(8:参考文献)の通り。


Analytical methods.------------------------------

Cell protein was determined by the method of Herbert et al. PO was measured by the bound hydroxylamine assay developed by Verstraete and Alexander.

細胞タンパク質はハーバードらの方法により決定した。

Free NH2OH was measured by the method of Magee and Burris after interfering nitrite had been destroyed by the addition of 0.5% sulfamic acid.

POは、VerstraeteとAlexanderにより発達した臨界ヒドロキシルアミン検定により測定した。

Nitrite and nitrate were assayed by, respectively, the diazotization method of Van't Riet et al. and the chromotropic acid method of Rand et al.

亜硝酸塩と硝酸塩は、Van't Rietらのジアゾ化法とRandらのクロモトロプ酸法などで試験した。

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A 回答 (1件)

The cells were washed twice with cold mineral salts mixture (lacking C and N compounds) and finally resuspended in an appropriate volume of the same mixture.



について。
この文は、
The cells were washed twice with cold mineral salts mixture (lacking C and N compounds).

The cells were finally resuspended in an appropriate volume of the same mixture.
というように、二つに分けることができます。

また、lacking C and N compoundsは、「CとNを含む化合物は入っていない」ということであり、
resuspendedは、一度回収したセルを液体に懸濁することを指します。
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