コンディショナルノックアウト法について

コンディショナルノックアウト法の概要と方法を詳しく教えてください。

No.1 回答者： MIYD 回答日時： 2008/07/26 08:14

詳しく知りたいのですね。

まずは、下記の論文とそれらに引用されている論文を読んでみましょう。
1: Related Articles, LinksSzymańska H.

[Genetically engineered mice: mouse models for cancer research]
Postepy Hig Med Dosw (Online). 2007 Oct 26;61:639-45. Review. Polish.
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The gamma/epsilon-secretase-derived APP intracellular domain fragments regulate p53.
Curr Alzheimer Res. 2007 Sep;4(4):423-6. Review.
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Genetic dissection of estrogen receptor signaling in vivo.
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Conditional and inducible gene recombineering in the mouse inner ear.
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Jun signalling in the epidermis: From developmental defects to psoriasis and skin tumors.
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New genetic tactics to model alveolar rhabdomyosarcoma in the mouse.
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Na(+)--Ca2+ exchange in the regulation of cardiac excitation-contraction coupling.
Cardiovasc Res. 2005 Aug 1;67(2):198-207. Review.
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The VHL tumor suppressor in development and disease: functional studies in mice by conditional gene targeting.
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Survey of genetically engineered mouse models for prostate cancer: analyzing the molecular basis of prostate cancer development, progression, and metastasis.
J Cell Biochem. 2005 Feb 1;94(2):279-97. Review.
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Analysis of mouse eye development with chimeras and mosaics.
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RNAi knock-down mice: an emerging technology for post-genomic functional genetics.
Cytogenet Genome Res. 2004;105(2-4):412-21. Review.
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Estrogen-regulated conditional oncoproteins: tools to address open questions in normal myeloid cell function, normal myeloid differentiation, and the genetic basis of differentiation arrest in myeloid leukemia.
Leuk Lymphoma. 2003 Jul;44(7):1131-9. Review.
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Gene targeting technology and advances in the pathophysiology of inflammation.
Pathology. 2002 Apr;34(2):109-14. Review.
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Genetically engineered mice for studies of stress-related clinical conditions.
J Psychiatr Res. 2002 Mar-Apr;36(2):53-76. Review.
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Genome engineering via homologous recombination in mouse embryonic stem (ES) cells: an amazingly versatile tool for the study of mammalian biology.
An Acad Bras Cienc. 2001 Sep;73(3):365-83. Review. Erratum in: An Acad Bras Cienc 2001 Dec;73(4):577-80.
PMID: 11600898 [PubMed - indexed for MEDLINE]17: Related Articles, LinksCohen-Tannoudji M, Babinet C.

Beyond 'knock-out' mice: new perspectives for the programmed modification of the mammalian genome.
Mol Hum Reprod. 1998 Oct;4(10):929-38. Review.
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Transgenic models in renal tubular physiology.
Exp Nephrol. 1998 Sep-Oct;6(5):438-46. Review.
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Genetic ablation in transgenic mice.
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PMID: 2699511 [PubMed - indexed for MEDLINE]